Home
PureCube 100 Co-NTA Agarose
The term Co-NTA refers to a copper Ion that has been coupled Nitrilotriacetic acid. Co-NTA can then be coupled to agarose or magnetic beads for IMAC (Immobilized Metal Chelate Affinity Chromatography). This is a purification method to obtain functional His-tagged protein. Cube Biotech offers multiple different Co-NTA based products for protein purification. Our PureCube 100 Co-NTA agarose consist of agarose resin beads with a diameter of 100 µm. They are used for the purification of active & stable His Tag protein from e.g. cell lysates. Our agarose resins are suited for batch spin columns and FPLC. Co-NTA provides purer protein eluates that Ni-NTA. Furthermore we recommend our PureCube Co-NTA resin with 40 µm diameter and for cases that require a drastically increased flow rate of the beads our Purecube XL Co-NTA agarose beads.
Our agarose resins are suited for batch spin columns and FPLC. Co-NTA provides purer protein eluates that Ni-NTA.
1. Protein Binding Capacity
The protein binding capacity is up to 30 mg/mL, as determined by purification of 6xHis-tagged GFP protein from E.coli cleared lysates, and quantified via spectrophotometry. Proteins are eluted with high purity.
2. Compatibility
PureCube 100 Co-NTA Agarose is very stable and can resist the following conditions in most situations: pH 3-13, 100% methanol, 100% ethanol, 8 M urea, 6 M guanidinium hydrochloride, 30% (v/v) acetonitrile, 10 mM DTT, 1 mM EDTA, 1 M NaOH.
Type: | Agarose |
Ligand: | NTA |
Coupled Ion: | Co2+ |
Usage | Specific binding and purification of 6x His-tagged proteins |
Specifity | Affinity to His-tagged proteins |
Binding capacity | >30 mg/mL |
Chelator stability | Stable in buffer containing 10 mM DTT and 1 mM EDTA |
Filling quantity | Delivered as a 50 % suspension |
Bead size | 40 μm |
Bead Ligand | Co-NTA |
Required equipment |
· Lysis Buffer · Wash Buffer · Elution Buffer · Ice bath · Refrigerated centrifuge for 50 mL tube (min 10,000 x g) · 50 mL centrifuge tube · Micropipettor and Micropipetting tips · Disposable gravity flow columns with capped bottom outlet, 2 ml · pH meter · End-over-end shaker · SDS-PAGE buffers, reagents and equipment Optional: Western Blot reagents and equipment |
Order number | Products | Bead size | Binding capacity | Shipment Temperature | Storage temperature | Usage | Price(yuan) |
74403 | PureCube 100 Co-NTA Agarose(10ml) | 100 | 30mg/mL | Ambient temperature | 4° C | PureCube 100 Ni-NTA Agarose is used for the purification of HIS-tagged recombinant proteins with high flow rate requirements; Max. Flow Rate: 1000cm/h; Maximum pressure: 300kPa; pH Stability: 3-13; Stable in 10mM DTT, 1mM EDTA, 1M NaOH ; Compared with 100 Ni-NTA, Co-NTA has higher specificity | 1227 |
74405 | PureCube 100 Co-NTA Agarose(50ml) | 100 | 30mg/mL | Ambient temperature | 4° C | 4992 | |
74410 | PureCube 100 Co-NTA Agarose(250ml) | 100 | 30mg/mL | Ambient temperature | 4° C | 21507 |
ask for price