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PureCube 100 Co-NTA Cartridge
The term Co-NTA refers to a copper Ion that has been coupled Nitrilotriacetic acid. Co-NTA can then be coupled to agarose or magnetic beads for IMAC (Immobilized Metal Chelate Affinity Chromatography). This is a purification method to obtain functional His-tagged protein. Cube Biotech offers multiple different Co-NTA based products for protein purification. FPLC is one of the best ways to purify proteins. Therefore we pre-filled cartridges / columns with our high quality PureCube 100 Co-NTA agarose resin beads. Co-NTA based purification products are the best way to obtain maximum purity of his tagged protein combined with high yields. Please refer the features table to confirm if the format of these cartridges / colums match your FPLC machinery e.g. ÄKTA. If these columns do not match your hardware try out our other 100 Co-NTA Cartridges for matching compability.
We pre-filled cartridges / columns with our high quality PureCube 100 Co-NTA agarose resin beads. Co-NTA based purification products are the best way to obtain maximum purity of his tagged protein combined with high yields.
1. Affinity Resin
PureCube 100 Co-NTA Agarose was developed for the affinity purification of proteins carrying a polyhistidine tag. This affinity chromatography matrix is based on a highly cross-linked, 6% agarose. The material is highly porous to allow for optimal protein interaction. PureCube 100 Agarose is also physically very stable, making it suitable for purification processes under low pressure with variable flow rates. The diameter of the beads is 50-150 µm, providing excellent flow behavior and a high degree of reproducibility between individual purification runs.
An NTA ligand is coupled to the agarose resin and carefully loaded with nickel ions to obtain a matrix with high specificity and binding capacity for histidine residues. The metal ion capacity is about Co µeqv Ni2+/mL. Other possible metal ions are Ni2+, Zn2+, Fe3+, Al3+, resulting in different affinities, e.g., for zincfinger proteins or phosphorylated proteins. If required, the nickel ions can be removed from the agarose matrix using five wash steps with 100 mM EDTA, and the matrix can be recharged with a different metal ion. Alternatively, please contact us for unloaded NTA agarose. NTA matrices charged with other metals are available upon request.
2. Protein Binding Capacity
PureCube 100 Compact Cartridges Co-NTA have a binding capacity of up to 20 mg/mL as determined by purification of 6xHis-tagged GFP protein from E.coli cleared lysates, and quantified via spectrophotometry. It should be considered that the dynamic binding capacity strongly correlates with flow rate and other parameters such as protein size and buffer conditions. It is recommended to use the lowest flow rate possible to achieve highest binding capacity.
3. Compatibility
For cleaning purposes, PureCube 100 Co-NTA Agarose is very stable and can resist the following conditions in most situations: All commonly used aqueous buffers, pH 2-14, 100% methanol, 100% ethanol, 8 M urea, 6 M guanidinium hydrochloride, 30% (v/v) acetonitrile, and up to 10 mM DTT.
Features - Regarding Purification capabilities.
Type | Cartridge |
Ligand | NTA |
Coupled Ion | Co2+ |
Usage | Specific binding and purification of 6x his-tagged proteins |
Specifity | Affinity to His-tagged proteins |
Binding capacity | >30 mg/mL |
Bead Ligand | Co-NTA |
Bead size | 100 μm(Agarose) |
Matrix | 6% highly cross-linked agarose |
Chelator stability | Stable in buffer containing 10 mM DTT and 1 mM EDTA |
ph Stability | 2-14 |
Features - Regarding Cartridge / Column itself.
Feature | PureCube Compact Cartridge 1 mL | PureCube Compact Cartridge 5 mL |
Bed Volume | 1 mL | 5 mL |
Dimensions: diameter X length (mm) | 8.0 x 35 | 17 x 35 |
Body material | Polypropylene | |
End Plug Material | Polypropylene | |
Inlet | 10-32 UNF female thread | |
Outlet | 10-32 UNF female thread | |
Max. Flow Rate | 6 mL/min | |
Order number | Products | Bead size | Protein Binding Capacity | Shipment Temperature | Storage temperature | Usage | Price(yuan) |
74602 | PureCube 100 Compact Cartridge Co-NTA(1×1ml) | 100 | 50mg | Ambient temperature | 4° C | Preassembled PureCube 100 Co-NTA Agarose; pH Stability: 3-13; Stable in 10mM DTT | 790 |
74604 | PureCube 100 Compact Cartridge Co-NTA(5×1ml) | 100 | 50mg | Ambient temperature | 4° C | 3108 | |
74606 | PureCube 100 Compact Cartridge Co-NTA(1×5ml) | 100 | 250mg | Ambient temperature | 4° C | 2534 | |
74608 | PureCube 100 Compact Cartridge Co-NTA(5×5ml) | 100 | 250mg | Ambient temperature | 4° C | 9710 |
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