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PureCube Ni-NTA MagBeads
The term Ni-NTA (Nickel NTA) refers to a nickel2+ ion that has been coupled to Nitrilotriacetic acid (NTA). Ni-NTA can then be coupled to agarose resin or magnetic beads for IMAC (Immobilized Metal Chelate Affinity Chromatography). This is a purification method to obtain functional His-tagged protein. The size of the used agarose resin beads or magnetic beads influences the flow rates and the protein yield. Our PureCube Ni-NTA MagBeads provide the highest standards for His-tag protein purification. With a fillung quantity of 25% high quality settled beads you get more than the market's average. For magnetic beads of a bigger size please consider our XL Ni-NTA Magbeads (~90 µm). For the magnetic separation process we offer our own PureCube MagBead separator for your convenience.
With a filling quantity of 25% high quality settled beads, you get more than the market's average. For the magnetic separation process we offer our own PureCube MagBead separator for your convenience.
1. Protein Binding Capacity
The protein binding capacity is 70 mg protein per mL of settled beads, as determined by purification of 6xHis-tagged GFP protein from E.coli cleared lysates, and quantified via spectrophotometry.
2. Compatibility
PureCube Ni-NTA MagBeads are very stable and can resist the following conditions in most situations: pH 2-4, 100% methanol, 100% ethanol, 8 M urea, 6 M guanidinium hydrochloride, 30% (v/v) acetonitrile.
Features - Regarding Purification capabilities.
Type | MagBeads |
Ligand | NTA |
Coupled Ion | Ni2+ |
Usage | Specific binding and purification of 6x his-tagged proteins |
Specifity | Affinity to His-tagged proteins |
Binding capacity | >80 mg/mL |
Bead Ligand | Ni-NTA |
Bead size | 30 µm(MagBeads) |
Filling quantity | Delivered as a 25 % suspension |
Chelator stability | Stable in buffer containing 10 mM DTT and 1 mM EDTA |
Order number Products Bead size Binding capacity Shipment Temperature Storage temperature Usage Price(yuan) 31201 PureCube Ni-NTA MagBeads(1mL) 30 80mg Ambient temperature 4° C Suitable for the isolation and purification of 6x His tagged proteins in dilute solution environment , or for pull-down experiments; Super high protein load; Excellent DTT and EDTA tolerance; pH Stability:2-10 676 31205 PureCube Ni-NTA MagBeads(5mL) 30 80mg Ambient temperature 4° C 2912 31225 PureCube Ni-NTA MagBeads(25mL) 30 80mg Ambient temperature 4° C 10869 31290 PureCube Ni-NTA MagBeads(4x25mL) 30 80mg Ambient temperature 4° C 34746
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