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PureCube NTA Agarose XL
PureCube NTA (nitrilotriacetic acid) XL agarose resin was developed for IMAC based protein purification and protein enrichment methods. Since the affinity of an NTA-metal-ion-complex depends on the loaded metal ion, we offer NTA XL agarose resin uncoupled to any metal ion for the case that we do not offer your desired NTA + metal ion combination. By providing our coupling protocols, feel free to create any NTA purification resin you desire. PureCube NTA resin is also available in regular bead sizes with a bead diameter of 40 µm and 100 µm sized . Alternatively we also offer NTA magnetic beads. Furthermore PureCube NTA agarose (40 µm) can also be obtained pre-filled into FPLC cartridges / columns.
1. Different metal ions confer different binding affinity and specificity
His-tag purification is based on ionic interactions of a coupled metal ion and the Poly-His-tag. Therefore other metal ions can also fullfill the function of Nickel. Figure 1 shows the most frequently used metal ions for His-tag purification. Depending on the used metal ion the balance between the specificity and the affinity of the metal ion to the His-tag shifts. Nickel is a balanced choice between both extremes. Copper has the highest affinity, but Cobalt is the choice for the most pure His-tag purifications.
Fig. 1: Depending on which metal ion is coupled to NTA the balance between protein yield and purification specificity shifts. Nickel is a very balanced choice between the two extremes.
Type | Agarose |
Ligand | NTA |
Coupled Ion | none |
Usage | For coupling of metal ions for corresponding purification and / or enrichment purposes |
Specifity | Varies |
Binding capacity | Depends on the loaded ion |
Purity | Depends on the loaded ion |
Bead Ligand | NTA |
Bead size | ~400 μm |
Matrix | 7.5% cross-linked agarose |
pH stability | 2-14 |
Other stabilities | 100% methanol, 100% ethanol, 8 M urea, 6 M guanidinium hydrochloride, 30% (v/v) acetonitrile |
Filling quantity | Delivered as a 50 % suspension |