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PureCube Ni-NTA MagBeads XL
The term Ni-NTA (Nickel NTA) refers to a nickel2+ ion that has been coupled to Nitrilotriacetic acid (NTA). Ni-NTA can then be coupled to agarose resin or magnetic beads for IMAC (Immobilized Metal Chelate Affinity Chromatography). This is a purification method to obtain functional His-tagged protein. The size of the used agarose resin beads or magnetic beads influences the flow rates and the protein yield. Our PureCube Ni-NTA XL MagBeads are magnetic beads with a diameter of ~90 µm. They are suited for the purification of His Tagged proteins. For our regular sized Ni-NTA MagBeads (~30 µm) please refer to this page. For the magnetic separation consider our PureCube MagBead separator.
1. Protein Binding Capacity
The protein binding capacity is ~40 mg protein per mL of settled beads, as determined by purification of 6xHis-tagged GFP protein from E.coli cleared lysates, and quantified via spectrophotometry.
2. Compatibility
PureCube Ni-NTA MagBeads XL are very stable and can resist the following conditions in most situations: pH 2-4, 100% methanol, 100% ethanol, 8 M urea, 6 M guanidinium hydrochloride, 30% (v/v) acetonitrile.
Features - Regarding Purification capabilities.
Type: | Magnetic Beads |
Ligand: | NTA |
Coupled Ion: | Ni2+ |
Usage | Specific binding and purification of 6x his-tagged proteins |
Specifity | Affinity to His-tagged proteins |
Binding capacity | >40 mg/mL |
Bead Ligand | Ni-NTA |
Bead size | 90 µm(XL MagBeads) |
Filling quantity | Delivered as a 25 % suspension |
Chelator stability | Stable in buffer containing 10 mM DTT and 1 mM EDTA |
pH stability | 2-14 |