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2023-04-21

PureCube HiCap StrepTactin MagBeads

Our PureCube StrepTactin® MagBeads is a suspension of 6% cross-linked magnetic agarose beads that is coupled to the StrepTactin protein. They enable protein yields of up to 7 mg protein per ml MagBeads.

They were developed for protein purification assays using the Strep-tag® using magnetic separation. PureCube StrepTactin® products can also be obtained as agarose resin. Furthermore, PureCube StrepTactin® MagBeads XL are also available for very vicious cell media.

For your convenience Cube Biotech also offers its own MagBead separator for use with our magnetic beads.


FEATURE

 

Usage

Highly specific affinity purification of Strep-(WSHPQFEK)-tagged proteins

Specificity

Affinity to Strep-tagged (WSHPQFEK) proteins

Binding capacity

>7 mg/ml

Bead Ligand

StrepTactin®

Bead size

30 μm

Matrix

6% cross-linked agarose

Filling quantity

Delivered as a 5 % suspension

Chemical stability

2% Tween 20, 2% Triton X-100, 2% IGEPAL® 630/Nonidet P40, n-Octyl-beta-D-glucopyranoside, 0,2% n-Nonyl-beta-D-gluyopyranoside, 0,35% N-Decyl-beta-D-maltoside, 2% Lauryl-sacrosine, 0,1% SDS, 0,3% CHAPS, 1M Guanidine HCl, 1mM PMSF, 10% Ethanol, 5M NaCl, 2M (NH4)SO4, 1M CaCl2, 25% Glycerol

Short-term storage

In equilibration buffer (see protocol)

Long-term storage

In 20% ethanol at 4°C


Citations

PURIFIED PROTEIN

YEAR

AUTHOR

NPTX1

2020

Sticco M.J., Palomino P.A.P., Lukacsovich D., Thompson B.L., Földy C., Ressl S., Martinelli D.C.

Sarm1

2021

Li Y., Pazyra-Murphy M.F., Avizonis D., de Sá Tavares Russo M., Tang S., Chen C-Y., Hsueh Y-P., Bergholz S.J., Jiang T., Zhao J. J:, Zhu J., Woo Ko K., Milbrandt J., DiAntonio A., Segal R.A.

NMNAT

2022

Dingwall C.B ., Strickland A., Yum S.W., Yim A.K., Zhu J., Wang P.L., Yamada Y., Schmidt R.E, Sasaki Y., Bloom A.J., DiAntonio A., Milbrandt J.

TIR domains and TIR domain-encoding proteins

2022

Weagley J.S., Zaydman M., Venkatesh S., Sasaki Y., Damaraju N., Yenkin A., Buchser W., Rodionov D.M. Osterman A., Ahmed T., Barratt M.J., DiAntonio A., Milbrandt J., Gordon J.I.


FAQ
Can I get the datasheet for the PureCube StrepTactin® Agarose?
Yes. Just click here to download it as a PDF File.


Is StrepTactin® the same as Streptavidin?
No, they are not. StrepTactin® is a bioengineered protein, originally based on Streptavidin. However, StrepTactin cannot bind to biotin, while Streptavidin cannot bind to the Strep-tag.


Can I regenerate & reuse your StrepTactin® beads?
Yes, you can! Simply follow our protocol.