PureCube HiCap StrepTactin MagBeads
They were developed for protein purification assays using the Strep-tag® using magnetic separation. PureCube StrepTactin® products can also be obtained as agarose resin. Furthermore, PureCube StrepTactin® MagBeads XL are also available for very vicious cell media.
For your convenience Cube Biotech also offers its own MagBead separator for use with our magnetic beads.
FEATURE |
|
Usage | Highly specific affinity purification of Strep-(WSHPQFEK)-tagged proteins |
Specificity | Affinity to Strep-tagged (WSHPQFEK) proteins |
Binding capacity | >7 mg/ml |
Bead Ligand | StrepTactin® |
Bead size | 30 μm |
Matrix | 6% cross-linked agarose |
Filling quantity | Delivered as a 5 % suspension |
Chemical stability | 2% Tween 20, 2% Triton X-100, 2% IGEPAL® 630/Nonidet P40, n-Octyl-beta-D-glucopyranoside, 0,2% n-Nonyl-beta-D-gluyopyranoside, 0,35% N-Decyl-beta-D-maltoside, 2% Lauryl-sacrosine, 0,1% SDS, 0,3% CHAPS, 1M Guanidine HCl, 1mM PMSF, 10% Ethanol, 5M NaCl, 2M (NH4)SO4, 1M CaCl2, 25% Glycerol |
Short-term storage | In equilibration buffer (see protocol) |
Long-term storage | In 20% ethanol at 4°C |
Citations
PURIFIED PROTEIN | YEAR | AUTHOR |
NPTX1 | 2020 | Sticco M.J., Palomino P.A.P., Lukacsovich D., Thompson B.L., Földy C., Ressl S., Martinelli D.C. |
Sarm1 | 2021 | |
NMNAT | 2022 | |
TIR domains and TIR domain-encoding proteins | 2022 |
FAQ
Can I get the datasheet for the PureCube StrepTactin® Agarose?
Yes. Just click here to download it as a PDF File.
Is StrepTactin® the same as Streptavidin?
No, they are not. StrepTactin® is a bioengineered protein, originally based on Streptavidin. However, StrepTactin cannot bind to biotin, while Streptavidin cannot bind to the Strep-tag.
Can I regenerate & reuse your StrepTactin® beads?
Yes, you can! Simply follow our protocol.