FEATURE

Usage

Highly specific affinity purification of Strep-(WSHPQFEK)-tagged proteins

Specificity

Affinity to Strep-tagged (WSHPQFEK) proteins

Binding capacity

>7 mg/ml

Bead Ligand

StrepTactin®

Bead size

30 μm

Matrix

6% cross-linked agarose

Filling quantity

Delivered as a 5 % suspension

Chemical stability

2% Tween 20, 2% Triton X-100, 2% IGEPAL® 630/Nonidet P40, n-Octyl-beta-D-glucopyranoside, 0,2% n-Nonyl-beta-D-gluyopyranoside, 0,35% N-Decyl-beta-D-maltoside, 2% Lauryl-sacrosine, 0,1% SDS, 0,3% CHAPS, 1M Guanidine HCl, 1mM PMSF, 10% Ethanol, 5M NaCl, 2M (NH4)SO4, 1M CaCl2, 25% Glycerol

Short-term storage

In equilibration buffer (see protocol)

Long-term storage

In 20% ethanol at 4°C

PURIFIED PROTEIN

YEAR

AUTHOR

NPTX1

2020

Sticco M.J., Palomino P.A.P., Lukacsovich D., Thompson B.L., Földy C., Ressl S., Martinelli D.C.

Sarm1

2021

Li Y., Pazyra-Murphy M.F., Avizonis D., de Sá Tavares Russo M., Tang S., Chen C-Y., Hsueh Y-P., Bergholz S.J., Jiang T., Zhao J. J:, Zhu J., Woo Ko K., Milbrandt J., DiAntonio A., Segal R.A.

NMNAT

2022

Dingwall C.B ., Strickland A., Yum S.W., Yim A.K., Zhu J., Wang P.L., Yamada Y., Schmidt R.E, Sasaki Y., Bloom A.J., DiAntonio A., Milbrandt J.

TIR domains and TIR domain-encoding proteins

2022

Weagley J.S., Zaydman M., Venkatesh S., Sasaki Y., Damaraju N., Yenkin A., Buchser W., Rodionov D.M. Osterman A., Ahmed T., Barratt M.J., DiAntonio A., Milbrandt J., Gordon J.I.