FEATURE

Usage

Highly specific affinity purification of Strep-(WSHPQFEK)-tagged proteins

Specificity

Affinity to Strep-tagged (WSHPQFEK) proteins

Binding capacity

>5 mg/ml

Bead Ligand

StrepTactin®

Bead size

100 μm

Matrix

6% cross-linked agarose

Recommended flow rates

0.25 - 1 ml/min

Max. flow rates

6 ml/min

Filling quantity

Delivered as a 50 % suspension

Chemical stability

2% Tween 20, 2% Triton X-100, 2% IGEPAL® 630/Nonidet P40, n-Octyl-beta-D-glucopyranoside, 0,2% n-Nonyl-beta-D-gluyopyranoside, 0,35% N-Decyl-beta-D-maltoside, 2% Lauryl-sacrosine, 0,1% SDS, 0,3% CHAPS, 1M Guanidine HCl, 1mM PMSF, 10% Ethanol, 5M NaCl, 2M (NH4)SO4, 1M CaCl2, 25% Glycerol

Short-term storage

In neutral buffer at 4°C

Long-term storage

In neutral buffer with 20% ethanol at 4°C

Required equipment

· Lysis Buffer

· Wash Buffer

· Elution Buffer (incl. Rho1D4 peptide)

· Ice bath

· Refrigerated centrifuge for 50 ml tube (min 10,000 x g)

· 50 mL centrifuge tube

· Micropipettor and Micropipetting tips

· Disposable gravity flow columns with capped bottom outlet, 2 ml

· pH meter

· End-over-end shaker

· SDS-PAGE buffers, reagents and equipment Optional: Western Blot reagents and equipment