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PureCube Co-NTA Agarose
The term Co-NTA refers to a copper Ion that has been coupled Nitrilotriacetic acid. Co-NTA can then be coupled to agarose or magnetic beads for IMAC (Immobilized Metal Chelate Affinity Chromatography). This is a purification method to obtain functional His-tagged protein. Cube Biotech offers multiple different Co-NTA based products for protein purification. Our PureCube Co-NTA agarose consist of small agarose resin beads with a diameter of 40 µm. Cobalt Ions that are coupled to NTA on the surface on the beads for purification of active His-tagged protein. Our agarose resins are suited for batch spin columns and FPLC. Due to their small size they are mechanically stable to a great extent. For Co-NTA agarose resin products that enable higher flow rates we recommend our PureCube 100 Co-NTA resin and for extreme cases our Purecube XL Co-NTA agarose beads.
Our Agarose resins are suited for batch spin columns and FPLC. Due to their small size they are mechanically stable to a great extent.
1. Protein Binding Capacity
The protein binding capacity is up to 30 mg/mL resin, as determined by purification of 6xHis-tagged GFP protein from E.coli cleared lysates, and quantified via spectrophotometry.
2. Compatibility
PureCube Co-NTA Agarose is very stable and can resist the following conditions in most situations: pH 2-14, 100% methanol, 100% ethanol, 8 M urea, 6 M guanidinium hydrochloride, 30% (v/v) acetonitrile.
Type | Agarose |
Ligand | NTA |
Coupled Ion | Co2+ |
Usage | Specific binding and purification of 6x His-tagged proteins |
Specifity | Affinity to His-tagged proteins |
Binding capacity | >30 mg/mL |
Chelator stability | Stable in buffer containing 10 mM DTT and 1 mM EDTA |
Filling quantity | Delivered as a 50 % suspension |
Bead size | 40 μm(Agarose) |
Bead Ligand | Co-NTA |
Required equipment |
· -Lysis Buffer · -Wash Buffer · -Elution Buffer · -Ice bath · -Refrigerated centrifuge for 50 mL tube (min 10,000 x g) · -50 mL centrifuge tube · -Micropipettor and Micropipetting tips · -Disposable gravity flow columns with capped bottom outlet, 2 ml · - pH meter · -End-over-end shaker · -SDS-PAGE buffers, reagents and equipment Optional: Western Blot reagents and equipment |
Order number | Products | Bead size | Binding capacity | Shipment Temperature | Storage temperature | Usage | Price(yuan) |
31403 | PureCube Co-NTA Agarose(10ml) | 40 | 30mg/mL | Ambient temperature | 4° C | Used for specific binding and purification of 6x His-tagged proteins; High specificity; pH Stability: 2-14; Max. Flow Rate: 6 mL/min | 1154 |
31405 | PureCube Co-NTA Agarose(50ml) | 40 | 30mg/mL | Ambient temperature | 4° C | 4524 | |
31410 | PureCube Co-NTA Agarose(250ml) | 40 | 30mg/mL | Ambient temperature | 4° C | 19594 |
相关产品：
1. 所有PureCube系列琼脂糖产品均提供用于自动液相色谱系统（如：FPLC）的预装柱（提供1mL和5mL）
2. 用于小规模His-tagged蛋白纯化或用于pull-down实验，我们推荐使用PureCube NTA Magbeads磁珠
3. 新型的INDIGO配基能够在高达20mM EDTA和DTT环境下表现优异。
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