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PureCube Co-NTA MagBead
The term Co-NTA refers to a copper Ion that has been coupled Nitrilotriacetic acid. Co-NTA can then be coupled to agarose or magnetic beads for IMAC (Immobilized Metal Chelate Affinity Chromatography). This is a purification method to obtain functional His-tagged protein. Cube Biotech offers multiple different Co-NTA based products for protein purification. For maximum purity of His-tagged protein Co-NTA is the best metal-ion + ligand option. As magnetic beads the purification process is easy to perform and does not require space-intensive tools. In case bigger MagBeads are desired please consider our For our Co-NTA MagBeads XL (~90 µm). Of course we are also offering our own PureCube MagBead separator for the separation process.
For maximum purity of His-tagged protein Co-NTA is the best metal-ion + ligand option. As magnetic beads the purification process is easy to perform and does not require space-intensive tools.
1. Protein Binding Capacity
The protein binding capacity is 30 mg protein per mL of settled beads, as determined by purification of 6xHis-tagged GFP protein from E.coli cleared lysates, and quantified via spectrophotometry.
2. Compatitibility
PureCube Co-NTA MagBeads are very stable and can resist the following conditions in most situations: pH 2-4, 100% methanol, 100% ethanol, 8 M urea, 6 M guanidinium hydrochloride, 30% (v/v) acetonitrile.
Features - Regarding Purification capabilities.
Type: | Magnetic Beads |
Ligand: | NTA |
Coupled Ion: | Co2+ |
Usage | Specific binding and purification of 6x His-tagged proteins |
Specifity | Affinity to His-tagged proteins |
Binding capacity | >30 mg/mL |
Bead Ligand | Co-NTA |
Bead size | 30 µm(MagBeads) |
Filling quantity | Delivered as a 25 % suspension |
Chelator stability | Stable in buffer containing 10 mM DTT and 1 mM EDTA |
pH stability | 2-14 |
Order number | Products | Bead size (μm) | Protein Binding Capacity | Shipment Temperature | Storage temperature | Usage | Price(yuan) |
31501 | PureCube Co-NTA MagBeads(1ml) | 30 | 30mg | Ambient temperature | 4° C | Suitable for the isolation and purification of 6x His-tagged proteins in dilute solution environment; or for pull-down experiments; High specificity; Excellent DTT and EDTA tolerance; pH Stability:2-10 | 738 |
31505 | PureCube Co-NTA MagBeads(5ml) | 30 | 30mg | Ambient temperature | 4° C | 3130 | |
31525 | PureCube Co-NTA MagBeads(25ml) | 30 | 30mg | Ambient temperature | 4° C | 11700 | |
31590 | PureCube Co-NTA MagBeads(4×25ml) | 30 | 30mg | Ambient temperature | 4° C | 37638 |
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