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PureCube NTA Agarose
PureCube NTA (nitrilotriacetic acid) agarose resin was developed for IMAC based protein purification and protein enrichment methods. Since the affinity of an NTA-metal-ion-complex depends on the loaded metal ion, we offer NTA agarose resin uncoupled to any metal ion for the case that we do not offer your desired NTA + metal ion combination. By providing our coupling protocols, feel free to create any NTA purification resin you desire. PureCube NTA resin is also available with a bead diameter of 100 µm or even XL sized for higher FPLC flow rates or as NTA magnetic beads. Furthermore PureCube NTA agarose can also be obtained pre-filled into FPLC cartridges / columns.
1. Protein Binding Capacity
The protein binding capacity strongly depends on the metal loaded NTA or 30 mg/ml for Co-NTA.
2. Compatibility
PureCube NTA Agarose is very stable and can resist the following conditions in most situations: pH 2-14, 100% methanol, 100% ethanol, 8 M urea, 6 M guanidinium hydrochlori acetonitrile.
3. Different metal ions confer different binding affinity and specificity
His-tag purification is based on ionic interactions of a coupled metal ion and the Poly-His-tag. Therefore other metal ions can also fullfill the function of Nickel. Figure 1 shows the most frequently used metal ions for His-tag purification. Depending on the used metal ion the balance between the specificity and the affinity of the metal ion to the His-tag shifts. Nickel is a balanced choice between both extremes. Copper has the highest affinity, but Cobalt is the choice for the most pure His-tag purifications.
Fig. 1: Depending on which metal ion is coupled to NTA the balance between protein yield and purification specificity shifts. Nickel is a very balanced choice between the two extremes.
Type | Agarose |
Ligand | NTA |
Coupled Ion | None |
Usage | For coupling of metal ions for corresponding purification and / or enrichment purposes |
Specifity | Varies |
Binding capacity | Depends on the loaded ion |
Purity | Depends on the loaded ion |
Bead Ligand | NTA |
Bead size | 40 μm |
Matrix | 7.5% cross-linked agarose |
Recommended FPLC flow rates | 0.5-2 mL/min |
Maximal FPLC flow rate | 6 mL/min |
pH stability | 2-14 |
Other stabilities | 100% methanol, 100% ethanol, 8 M urea, 6 M guanidinium hydrochloride, 30% (v/v) acetonitrile |
Filling quantity | Delivered as a 50 % suspension |
Order number | Products | Bead size | Protein Binding Capacity | Shipment Temperature | Storage temperature | Usage | Price(yuan) |
31703 | PureCube NTA Agarose(10ml) | 40 | Depending on the binding metal | Ambient temperature | 4° C | Use for highly specific binding and purification of 6x His-tagged proteins; Stable in buffer containing 10 mM DTT and 1 mM EDTA; pH Stability: 2-14; Max. Flow Rate: 6 mL/min | 1071 |
31705 | PureCube NTA Agarose(50ml) | 40 | Ambient temperature | 4° C | 4264 | ||
31710 | PureCube NTA Agarose(250ml) | 40 | Ambient temperature | 4° C | 18450 | ||
31712 | PureCube NTA Agarose(500ml) | 40 | Ambient temperature | 4° C | 33010 |
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